Synthesis/biological evaluation of hydroxamic acids and their prodrugs as inhibitors for Botulinum neurotoxin A light chain

Bioorg Med Chem. 2014 Feb 1;22(3):1208-17. doi: 10.1016/j.bmc.2013.11.053. Epub 2013 Dec 8.

Abstract

Botulinum neurotoxin A (BoNT/A) is the most potent toxin known. Unfortunately, it is also a potential bioweapon in terrorism, which is without an approved therapeutic treatment once cellular intoxication takes place. Previously, we reported how hydroxamic acid prodrug carbamates increased cellular uptake, which translated to successful inhibition of this neurotoxin. Building upon this research, we detail BoNT/A protease molecular modeling studies accompanied by the construction of small library of hydroxamic acids based on 2,4-dichlorocinnamic hydroxamic acid scaffold and their carbamate prodrug derivatization along with the evaluation of these molecules in both enzymatic and cellular models.

Keywords: Botulinum neurotoxin; Carbamate prodrug; Hydroxamic acid; Protease inhibitor; SNAP-25; SNARE; Zinc-dependent metalloprotease.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Botulinum Toxins, Type A / antagonists & inhibitors*
  • Chemistry Techniques, Synthetic
  • Hydroxamic Acids / chemical synthesis
  • Hydroxamic Acids / chemistry*
  • Hydroxamic Acids / pharmacology
  • Models, Molecular
  • Prodrugs / chemical synthesis
  • Prodrugs / chemistry*
  • Prodrugs / pharmacology*
  • Protease Inhibitors / chemistry
  • Protease Inhibitors / pharmacology*
  • Small Molecule Libraries / chemical synthesis
  • Small Molecule Libraries / chemistry*
  • Small Molecule Libraries / pharmacology*

Substances

  • Hydroxamic Acids
  • Prodrugs
  • Protease Inhibitors
  • Small Molecule Libraries
  • Botulinum Toxins, Type A